Apart from our regular monitoring of eggs we are also interest in running some experiments to help accuracy.
If anyone has the time here are some ideas for experiments...
- Take a single large sample enough to do several 1gm fecalyzers.
- Prepare fecalyzer egg with float 10 min wait and count.
- Prepare fecalyzer egg with float 15 min wait and count.
- Prepare fecalyzer egg with float 20 min wait and count.
Record your counts with conditions.
At the moment we think we are seeing more stable repeatable results using 20 mins wait.
I suggest we classify stool sample as firm/soft/runny.
It will be interesting to graph egg count for each category (ie. 2 or 3 graphs instead of 1 all inclusive graph).
Does storing the stool in the fridge (in a seal container wrapped in gladwrap) for a number of days change the egg count results.
A stool sample that is measured fresh. Then at 2 hour intervals. Any variation in egg count over time.
Fecalyzer Cage Turn
Using a single stool sample, experiment with how many times you turn the cage. 10, 15, 20, 25, 30, 35. Is egg count effected? This may over lap with the "Count Delay" experiment.
Idea #1: When topping up the faecal mixture use a set amount of liquid. I would be tempted to actually take a fixed volume from the top of the solution using a pipette, rather than laying a slide on the meniscus. Knowing the absolute volume of total float solution and the volume on the slide allows you to calculate more accurately the total number in the faeces. It also means the volume taken for analysis can be diluted to give optimum numbers of eggs on the slide for accurate counting.
Idea #2: After grabbing a sample from the fecalyzer, how many eggs are left in the remaining sludge?
Idea #3: Has anyone taken samples over a period of time to see how quickly the ova float? This is probably the "Variable Wait" experiment above.
Idea #4: Has anyone looked at the difference between the different float solutions?